The Childrens Hospital of Los Angeles hESC Facility

The Childrens Hospital of Los Angeles hESC Facility

Funding Type: 
Shared Labs
Grant Number: 
CL1-00507-1.2
Award Value: 
$1,750,259
Stem Cell Use: 
iPS Cell
Embryonic Stem Cell
Status: 
Active
Public Abstract: 
Statement of Benefit to California: 
Progress Report: 

Year 1

The renovation and equipping of the 3,000sqft California Institute for Regenerative Medicine sponsored Childrens Hospital Los Angeles Shared Stem Facility was completed in September 2009. The facility has four tissue culture suites with eleven clean tissue culture work stations, and associated equipment. The facility also has a large capacity for frozen sample storage, specialized microscopes and other equipment for stat-of-the-art molecular analyses of human stem cells. Since September 2009, the core staff has calibrated and monitored the laboratory environment and instrumentation, trained research personnel in the use of the shared equipment and performed on-going maintenance of the facility. The staff has also performed one-on-one training of personnel in the generation of human iPSC, genetic modification of human pluripotent stem cells, and routine culture of human embryonic stem cells. The CHLA Stem Cell Core staff has also maintained stocks of monitored and quality tested human embryonic stem cells including weekly, bi-weekly and monthly quality control analyses on the cells. During the first year of operation, 34 quality controlled human embryonic stem cell cultures were distributed to researchers at CHLA the first year of operation. These investigators have used these cells to develop models and study a variety of diseases including pediatric cancers (neuroblastoma, Ewings-sarcoma, retinoblastoma, lymphoma) and genetic diseases such as Cystic Fibrosis. Other researchers used the stem cells to study the genes and proteins that enable stem clel growth and expansion as well as differentiation. This provision of quality controlled stocks of human stem cells to investigators has decreased the amount of time individual research laboratories spend performing routine cell culture maintenance and quality control. In turn, this will increase the rate and efficiency of stem cell research at CHLA.

Year 2

The CIRM sponsored Children’s Hospital Los Angeles Shared Stem Cell Facility underwent a leadership change and new personnel were hired in January 2011. The facility was reorganized and resupplied to support an increase in research utilization within the space. The staff has been providing hPSCs to researchers interested in working the pluripotent stem cell field, as well as protocols, education, consultation, protocol development, and hPSC culture technique training for those interested in entering the field. The stem cell core has been working with biotech industry to provide educational seminars to the researchers of CHLA on applications of tools in stem cell research. Research occurring in the Shared facility has increased since January 2011. Studies using the facility now include the development of disease models for retinoblastoma and Ewings sarcoma, including the study of genes and pathways, such as in retinoblastoma tumorigenesis, sympathoadrenal differentiation, and the role of the Wnt signaling pathway in hESCs. Additional studies are being developed include a disease model of intestinal development and inflammation, and stable gene knock-downs in hPSCs. The Shared facility has also been involved in assisting with developing a potential stem cell mediated therapy.

Year 3

Over the last reporting period, the Children's Hospital Los Angeles Shared Research Lab (SRL) has been involved with human pluripotent stem cell research, adult stem cell research, and mouse embryonic stem cell research involving CHLA, USC, and the City of Hope research institutions. We are also participating in collaborative research with the USC Stem Cell Core facility on projects that aims to understand the biological properties of hPSC and to develop improved technologies for working with these cells. The CHLA (SRL) provides comprehensive support for researchers in the Los Angeles area, offering services including quality controlled cells and reagents, shared equipment, laboratory space, technical assistance, consultation, biobanking and training. Although the CHLA SRL does not run a formal CIRM funded techniques course, CHLA in collaboration with Dr. Victoria Fox from the USC Stem Cell Core Facility offered a two and a half day workshop for CHLA investigators wishing to learn pluripotent stem cell culture techniques. We plan to offer this course a twice a year--open to CHLA and non-CHLA researchers. The SRL also provided significant one-on-one training to CHLA researchers and support for students attending two newly established high school research program at USC: the EiHS Summer Program in Stem Cell Research and the CIRM STAR program, which is supported by a CIRM funded creativity award. We will continue to mentor and support this student though the next academic year. The stem cell core was instrumental in mentoring and supporting CIRM bridges students during the last year. We provided support, knowledge, and education regarding their projects and technical assistance with cell expansion and biobanking.

Year 4

Over the last reporting period, the Children's Hospital Los Angeles Shared Research Lab (SRL) has been involved with human pluripotent stem cell research and rodent embryonic stem cell research with CHLA and USC research institutions. We are also participating in collaborative research with the USC Stem Cell Core facility on projects that aims to understand the biological properties of hPSC and to develop improved technologies for working with these cells. The CHLA (SRL) provides comprehensive support for researchers in the Los Angeles area, offering services including quality controlled cells and reagents, shared equipment, laboratory space, technical assistance, consultation, biobanking and training. Although the CHLA SRL does not run a formal CIRM funded techniques course, CHLA in collaboration with Dr. Victoria Fox from the USC Stem Cell Core Facility offered a two and a half day workshop for CHLA investigators wishing to learn pluripotent stem cell culture techniques. We plan to offer this course a twice a year--open to CHLA and non-CHLA researchers. We also offer a 2.5 day basic cell culture course for investigators needing an introduction to the techniques required to work with these systems. We plan to offer this course at twice a year, and as needed to CHLA and non-CHLA researcher. The SRL also provided significant one-on-one training to CHLA researchers and mentored a student attending the USC Early Investigators High School Summer Program in Stem Cell Research and the CIRM STAR program, which is supported by a CIRM funded creativity award. The stem cell core was instrumental in mentoring and supporting CIRM bridges students during the last year. We provided support, knowledge, and education regarding their projects and technical assistance with cell expansion.

Year 5

Over the last reporting period, the Children's Hospital Los Angeles Shared Research Lab (SRL) has been involved with human pluripotent stem cell research and rodent embryonic stem cell research with CHLA and USC research institutions. The CHLA (SRL) provides comprehensive support for researchers in the Los Angeles area, offering services including quality controlled cells and reagents, shared equipment, laboratory space, technical assistance, consultation, biobanking and training. Although the CHLA SRL does not run a formal CIRM funded techniques course, CHLA offers one on one training to investigators wishing to learn pluripotent stem cell culture techniques. We also offer a 2.5 day basic cell culture course for investigators needing an introduction to the techniques required to work with these culturing systems. The stem cell core was instrumental in mentoring and supporting CIRM bridges students during the last year. We provided support, knowledge, and education regarding their projects and technical assistance with cell expansion.

© 2013 California Institute for Regenerative Medicine