We have developed a synthetic polymer interface for the long-term self-renewal of human embryonic stem cells (hESCs) in defined media. We successfully cultured hESCs on hydrogel interfaces of aminopropylmethacrylamide (APMAAm)…
Chromosomal translocation is often associated with congenital genetic disorders, infertility, and cancers. The lack of cellular and animal models for chromosomal translocations, however, has hampered our ability to understand the…
We describe a novel model for investigation of genetically normal human osteoblasts in culture. Availability of culture models for molecular investigation of genetically normal human osteoblasts is important because differences…
The rat is the preferred animal model in many human health-related research fields. The availability of rat embryonic stem (ES) cells has enabled the creation of rat models with a…
Programmed cell death (PCD) occurs in adults to maintain normal tissue homeostasis and during embryological development to shape tissues and organs. During development, toxic chemicals or genetic alterations can cause…
We describe here a detailed protocol for generating gene knockout rats by homologous recombination in embryonic stem (ES) cells. This protocol comprises the following procedures: derivation and expansion of rat…
Stem cells are the primitive cells that give rise to the different tissue types in the body. In a way, stem cells are the universal cells from which all cells…
Embryonic stem (ES) cells are derived from blastocyst-stage embryos and are thought to be functionally equivalent to the inner cell mass, which lacks the ability to produce all extraembryonic tissues.…
