Name:
Institution:
Type:
PI
Grant Award Details
Scalable, Defined Production of Oligodendrocyte Precursor Cells to Treat Neural Disease and Injury
Grant Type:
Grant Number:
DISC2-08982
Project Objective:
Develop optimized, defined, scalable 3D process for hESC-derived OPC
Investigator:
Disease Focus:
Neurological Disorders
Spinal Cord Injury
Human Stem Cell Use:
Embryonic Stem Cell
iPS Cell
Award Value:
$1,627,099
Status:
Closed
Progress Reports
Reporting Period:
Year 2/NCE period
View Report
Grant Application Details
Application Title:
- Scalable, Defined Production of Oligodendrocyte Precursor Cells to Treat Neural Disease and Injury
Public Abstract:
Research Objective
The goal of this proposal is to develop an optimized, scalable process to manufacture high quality oligodendrocyte precursor cells (OPCs) from human pluripotent stem cells for treating human disease.
Impact
OPCs have therapeutic potential for spinal cord injury, restoration of cognitive function after cancer radiation therapy, inherited demyelinating disease, and potentially multiple sclerosis.
Major Proposed Activities
- To engineer human embryonic stem cell lines with fluorescent protein reporters to quantify differentiation into oligodendrocyte precursor cells (OPCs).
- To use a high throughput system to screen thousands of cell culture conditions and thereby optimize a chemically-defined three-dimensional culture for differentiation into OPCs.
- To validate the capacity of the differentiated oligodendrocyte precursor cells to remyelinate neurons in culture and in the nervous system.
- To scale up this cell manufacturing system in a bioreactor for future translation towards preclinical and clinical studies.
Statement of Benefit to California:
This proposal will accelerate the development of a stem cell therapy to treat patients with demyelinating conditions, a serious unmet medical need. Also, the PI has a strong record of translating research towards clinical development within industry, particularly within California. Finally, this project will expose young scientists within a large stem cell center to highly interdisciplinary training at the interface of science and engineering, thereby enhancing our California workforce.
Publications
- Stem Cell Reports (2017) Defined and Scalable Differentiation of Human Oligodendrocyte Precursors from Pluripotent Stem Cells in a 3D Culture System. (PubMed: 28552605)
