Embryonic Animal Model for Generation of Retinal/RPE Precursors from Human Stem Cells
Age-related macular degeneration (AMD) is the leading cause of blindness in California and the United States, for which there is no cure. It has devastating effects on the quality of life as it makes it impossible for affected individuals to read and drive, and has become a major public health concern in the rapidly expanding aging population of California. AMD is particularly amenable to potential stem cell-based therapy because the affected areas of retinal pigment epithelium (RPE, the tissue primarily affected by the disease) and the overlying retina are very small, may be directly visualized, and transplantation of stem cells could be performed by direct injections using currently available surgical techniques.
A number of recent studies suggest a great potential for using various populations of stem cells to improve the morphological and functional capacity of RPE and the retina. However, we are yet to develop a stem cell-based approach for the management of specific retinal diseases such as AMD, and to establish clinically acceptable treatment protocols. In addition to human embryonic stem cells, adult, patient-own stem cells may provide the safest approach in patients with AMD at least for the near future. This implies using adult stem cells obtained from an easily accessible and abundant tissue source such as bone marrow from the same patient, which greatly increases their immune compatibility and overall safety. Following their isolation, these cells may be injected into the eyes of patients with age-related macular degeneration into the areas affected by the disease.
There are two major obstacles that prevent successful therapeutic retinal/RPE regeneration in patients with AMD using stem cells due to the wide developmental gap between the immature stem cells and mature adult recipient tissues. Thus, when transplanted into adult tissues, these immature cells have a relatively low potential for developing into adult cells bearing the desired retinal/RPE function and have relatively low efficiency to engraft within adult microenvironment.
We propose to develop a robust, convenient and rapid “natural incubator” approach using chicken embryos for partially differentiating human stem cells of various origins into high-quality mature precursors of retinal photoreceptors and RPE cells suitable for their therapeutic transplantation in adult ocular tissues. Embryonic chick eye provides an in vivo habitat for the donor stem cells to follow the natural developmental program. We also propose to modulate interactions between donor stem cells and the host ocular microenvironment to enhance the efficiency of stem cell incorporation using laser irradiation. Our proposed studies provide the foundation for future development of an efficient stem cell-based management strategy for AMD and, therefore, have important therapeutic implications for the leading cause of blindness in California and the United States.
My proposed research focuses on the development of stem cell-based therapeutic approaches to age-related macular degeneration (AMD) and other diseases in which the deterioration of retinal pigment epithelium (RPE) and retina plays a significant role. It will benefit the citizens of the state of California in several important ways. AMD is the leading cause of blindness in the United States, and in California, and it is currently incurable. It has devastating effects on quality of life and independence, and is becoming a major public health concern as California’s population ages, and the state’s older citizens live longer, and require more assistance in the activities of daily living. The socioeconomic and emotional impact of AMD is tremendous because the disease affects central vision, limiting the ability of those who suffer from it to drive or read. Identifying a successful treatment would allow tens of thousands of elderly Californians to enjoy a more functional, productive, and enjoyable years beyond retirement.
Several studies have suggested the great therapeutic potential of various populations of embryonic and adult stem cells derived from the bone marrow for regeneration of retina and/or RPE. Stem cell transplantation has already received considerable attention as a novel neurotherapeutic strategy, but so far, little work has been devoted to stem cell therapies for specific retinal disorders. We have yet to develop a stem cell approach for the management of specific retinal diseases like AMD, or to establish clinically acceptable treatment protocols. While human embryonic stem cells have high regeneration potential, patient-specific transplantation of adult stem cells obtained from patient’s own bone marrow has certain practical advantages. Most importantly it eliminates the need for immunosuppressant drugs necessary in transplantation of cells or tissue from another individual, and carries no risk of tumor formation. The research proposed in the current application to the California Institute for Regenerative Medicine will utilize the chick embryonic ocular model to lay the groundwork for the use of the various populations of human stem cells as a potential therapy for the blinding retinal degenerative and age-related disorders. This will benefit California’s citizens by speeding our progress towards a safe and effective treatment for AMD and other related diseases affecting vision.