Efficient Scale-Up Cryopreservation of Human Pluripotent Cells and Their Progeny
Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) have tremendous potential for basic research and applied applications. However, a severe limitation is currently inefficient recovery after cell freezing. Cell freezing is essential for preserving cells (e.g. banking) for future use. This proposal seeks to apply modern cryobiological principles and methods to efficient preseration of stem cells. The results will accelerate research and large scale applications.
This proposal is a multidisciplinary collaboration to address a common problem in the stem cell field. Preservation of stem cells and their differentiated derivatives is essential but currently inefficient. or, in cases of some differentiated derivatives, have not been developed at all .The benefits to California include:
1. The new technology will find application in both basic and applied research settings across the state. All institutions doing stem cell research deal with preserved cells, so improving the protocols will benefit all stem cell scientits by accelerating their research.
2. The benefits extend to companies, such as pharmaceutical and biotech companies, since they rely on preserved samples of cells for their products and services.
3. Bringing the diverse people together (cryobiologists, engineers, and stem cell biologists) to address a stem cell problem forges new links in the academic community that should be capable of opening new areas of research. These new areas of research will be a important legacy of the stem cell initiative and will invigorate academic research.