From MEFs to Matrigel 2: splitting hESCs from MEFs onto Matrigel.

Journal: 
J Vis Exp
Publication Year: 
2008
Authors: 
Ivan Khvorostov
Jin Zhang
Michael Teitell
PubMed link: 
19066543
Public Summary: 
This video demonstrates how to grow human embryonic stem cells (hESCs) on mouse embryonic fibroblast (MEF) feeder cells, how to passage hESCs from MEF plates to feeder cell-free Matrigel plates.
Scientific Abstract: 
This video demonstrates how to grow human embryonic stem cells (hESCs) on mouse embryonic fibroblast (MEF) feeder cells, how to passage hESCs from MEF plates to feeder cell-free Matrigel plates.