From MEFs to Matrigel 3: passaging hESCs from Matrigel onto Matrigel.

Journal: 
J Vis Exp
Publication Year: 
2008
Authors: 
Jin Zhang
Ivan Khvorostov
Michael Teitell
PubMed link: 
19066542
Public Summary: 
This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated.
Scientific Abstract: 
This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated.