Tissue-Resident Macrophages Self-Maintain Locally throughout Adult Life with Minimal Contribution from Circulating Monocytes.

Journal: 
Immunity
Publication Year: 
2013
Authors: 
Daigo Hashimoto
Andrew Chow
Clara Noizat
Pearline Teo
Mary Beth Beasley
Marylene Leboeuf
Christian D Becker
Peter See
Jeremy Price
Daniel Lucas
Melanie Greter
Arthur Mortha
Scott W Boyer
E Camilla Forsberg
Masato Tanaka
Nico van Rooijen
Adolfo Garcia-Sastre
E Richard Stanley
Florent Ginhoux
Paul S Frenette
Miriam Merad
PubMed link: 
23601688
Public Summary: 
Scientific Abstract: 
Despite accumulating evidence suggesting local self-maintenance of tissue macrophages in the steady state, the dogma remains that tissue macrophages derive from monocytes. Using parabiosis and fate-mapping approaches, we confirmed that monocytes do not show significant contribution to tissue macrophages in the steady state. Similarly, we found that after depletion of lung macrophages, the majority of repopulation occurred by stochastic cellular proliferation in situ in a macrophage colony-stimulating factor (M-Csf)- and granulocyte macrophage (GM)-CSF-dependent manner but independently of interleukin-4. We also found that after bone marrow transplantation, host macrophages retained the capacity to expand when the development of donor macrophages was compromised. Expansion of host macrophages was functional and prevented the development of alveolar proteinosis in mice transplanted with GM-Csf-receptor-deficient progenitors. Collectively, these results indicate that tissue-resident macrophages and circulating monocytes should be classified as mononuclear phagocyte lineages that are independently maintained in the steady state.