Rapid and cost-effective gene targeting in rat embryonic stem cells by TALENs.

Journal: 
J Genet Genomics
Publication Year: 
2012
Authors: 
Chang Tong
Guanyi Huang
Charles Ashton
Hongping Wu
Hexin Yan
Qi-Long Ying
PubMed link: 
22749015
Public Summary: 
Scientific Abstract: 
The rat is the preferred animal model in many areas of biomedical research and drug development. Genetic manipulation in rats has lagged behind that in mice due to the lack of efficient gene targeting tools. Previously, we generated a knockout rat via conventional homologous recombination in rat embryonic stem (ES) cells. Here, we show that efficient gene targeting in rat ES cells can be achieved quickly through transcription activator-like effector nuclease (TALEN)-mediated DNA double-strand breaks. Using the Golden Gate cloning technique, we constructed a pair of TALEN targeting vectors for the gene of interest in 5 days. After gene transfection, the targeted rat ES cell colonies were isolated, screened, and confirmed by PCR without the need of drug selection. Our results suggest that TALEN-mediated gene targeting is a superior means of establishing genetically modified rat ES cell lines with high efficiency and short turnaround time.