Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells.

Journal: 
Mol Biosyst
Publication Year: 
2009
Authors: 
Jeffrey K Takimoto
Katrina L Adams
Zheng Xiang
Lei Wang
PubMed link: 
19668857
Public Summary: 
Scientific Abstract: 
Optimizing the anticodon recognition between orthogonal tRNA and synthetase significantly increased the incorporation efficiencies of various unnatural amino acids in mammalian cells, and the enhanced incorporation enabled efficient photocrosslinking of interacting proteins in mammalian cells.