Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells.

Mol Biosyst
Publication Year: 
Jeffrey K Takimoto , Katrina L Adams , Zheng Xiang , Lei Wang
Public Summary: 
Scientific Abstract: 
Optimizing the anticodon recognition between orthogonal tRNA and synthetase significantly increased the incorporation efficiencies of various unnatural amino acids in mammalian cells, and the enhanced incorporation enabled efficient photocrosslinking of interacting proteins in mammalian cells.

© 2013 California Institute for Regenerative Medicine