A bioinformatic assay for pluripotency in human cells.

Journal:

Nat Methods

Publication Year:

2011

Authors:

F J Muller

B M Schuldt

R Williams

D Mason

G Altun

E P Papapetrou

S Danner

J E Goldmann

A Herbst

N O Schmidt

J B Aldenhoff

L C Laurent

J F Loring

PubMed link:

Read the abstract

Funding Grants:

Center for hESC Research

The Stem Cell Matrix: a map of the molecular pathways that define pluripotent cells

Ensuring the safety of cell therapy: a quality control pipeline for cell purification and validation

Public Summary:

Pluripotent stem cells (PSCs) are defined by their potential to generate all cell types of an organism. The standard assay for pluripotency of mouse PSCs is cell transmission through the germline, but for human PSCs researchers depend on indirect methods such as differentiation into teratomas in immunodeficient mice. Here we report PluriTest, a robust open-access bioinformatic assay of pluripotency in human cells based on their gene expression profiles.

Scientific Abstract:

Pluripotent stem cells (PSCs) are defined by their potential to generate all cell types of an organism. The standard assay for pluripotency of mouse PSCs is cell transmission through the germline, but for human PSCs researchers depend on indirect methods such as differentiation into teratomas in immunodeficient mice. Here we report PluriTest, a robust open-access bioinformatic assay of pluripotency in human cells based on their gene expression profiles.