The University of California: irvine Regional Human Embryonic Stem Cell Shared Research Laboratory and Stem Cell Techniques Course

The University of California: irvine Regional Human Embryonic Stem Cell Shared Research Laboratory and Stem Cell Techniques Course

Funding Type: 
Shared Labs
Grant Number: 
CL1-00520-1.2
Award Value: 
$3,072,500
Stem Cell Use: 
Embryonic Stem Cell
iPS Cell
Status: 
Active
Public Abstract: 
Statement of Benefit to California: 
Progress Report: 

Year 1

During the reporting period, the UC Irvine Regional Human Embryonic Stem Cell Shared Research Laboratory and Stem Cell Techniques Course continued to train scientists in the use and application of human pluripotent stem cells. We continued to train students, technicians, postdoctoral fellows and faculty in the use of Human Embryonic Stem Cells (hESCs). These individuals have come from all over the world. In the reporting period we trained 30 individuals in our training course. Most of the individuals were from UC Irvine, one from Kyoto University in Japan, one from West Coast University and two students from the newl-integrated Bridges to Stem Cell Research from Cal State Long Beach. These courses are tremendously popular and are oversubscribed. The training provided by the Stem Cell Techniques Course has enabled enormous scientific progress in a variety of areas related to the mission of the California Institute for Regenerative Medicine (CIRM). Scientists trained in the course have gone on to do groundbreaking research in many areas including Alzheimer’s disease, glioblastoma, multiple sclerosis, spinal cord injury and many others. In addition the Shared Research Laboratory has continued to serve as the training site for the Stem Cell Emphasis Track of the UC Irvine Masters Program in Biotechnology. This program was designed to train technicians for the biotechnology industry. Because of the paucity of technicians trained in the use of hESCs and other pluripotent stem cells (PSCs), this is a much sought after workforce. The facilities and equipment provided under this CIRM award have also been made available to many investigators across the university for carrying out stem cell-related research. In addition to the research mission of the program the Shared Research Laboratory has provided outreach to the community and education about stem cell research to members of the public. The staff of the Shared Research Laboratory has regularly provided tours to visitors from around the world as well as members of the local community. These include local high school teachers, local teaching organizations such as Vital Link and the Coastline Regional Occupational Program, members of the business community, philanthropic donors and our local Congressional representatives. The outreach activities of the Shared Research Laboratory serve to educate the public about our work and to address the needs of our local patient communities. They in turn provide important advocacy within the local community and with our representatives at the state and federal level. In short, the Human Embryonic Stem Cell Shared Research Laboratory and Stem Cell Techniques Course continues to serve a vital function within the stem cell research program at UC Irvine and within the larger research community in Orange County.

Year 2

The UC Irvine Regional Human Embryonic Stem Cell Shared Research Laboratory and Stem Cell Techniques Course continues to be an invaluable element in supporting the mission of the Sue and Bill Gross Stem Cell Research Center to develop new treatments for human disease. The completion of Gross Hall: A CIRM Institute in May of 2010 allowed us to transition the activities of the lab and its associated teaching into a new world-class facility designed to be the hub of all stem cell research on the UC Irvine campus and in the region. Over the last year we have continued to train many students in stem cell biology at the didactic and practical levels and enabled their entry into the stem cell field. In addition, we have continued to support the research activities of many investigators on the Irvine campus who are already working in the field of regenerative medicine. During this period we have developed novel training methods and made all of our state-of-the-art equipment available to any researchers carrying out research relevant to the CIRM mission. Because of our new facility, we have been able to offer more hands-on training for the many specialized pieces of equipment in the building as well as providing didactic training courses for techniques and methods in the stem cell field. The facilities and courses continue to represent an essential element in the training of our fellows on the CIRM Training Grant II, other graduate students , postdocs, technicians and faculty on the Irvine campus and trainees on the CIRM Bridges programs. The Shared Research Laboratory also continues to host many outreach activities for members of the public. At these events, stem cell research is explained to a lay audience and members of the public are given an opportunity to see stem cells and learn about the incredible opportunities they provide for developing new treatments for human diseases and injuries. Many groups have visited the facility including high school kids, teachers, patients, patient advocates and caregivers as well as donors and potential donors. Each of these groups is inspirational in their own way and they are also inspired by the science and the potential of stem cell research. In short, the UC Irvine Regional Human Embryonic Stem Cell Shared Research Laboratory and Stem Cell Techniques Course continues to be an essential element in the success of the Sue and Bill Gross Stem Cell Research Center.

Year 3

The UC Irvine Regional Human Embryonic Stem Cell Shared Research Laboratory and Stem Cell Techniques Course continues to be an invaluable element in supporting the mission of the Sue and Bill Gross Stem Cell Research Center to develop new treatments for human disease. Over the last year we have continued to train many students in stem cell biology at the didactic and practical levels and enabled their entry into the stem cell field. In addition, we have continued to support the research activities of many investigators on the Irvine campus who are already working in the field of regenerative medicine. During this period we have developed novel training methods and made all of our state-of-the-art equipment available to any researchers carrying out research relevant to the CIRM mission. In the past year we have also instigated a new training course designed to train researchers in methods of cell transplantation, a pre-requisite for translating stem cell research into clinical use. Because of our new facility, we have been able to offer more hands-on training for the many specialized pieces of equipment in the building as well as providing didactic training courses for techniques and methods in the stem cell field. The facilities and courses continue to represent an essential element in the training of our fellows on the CIRM Training Grant II, other graduate students , postdocs, technicians and faculty on the Irvine campus and trainees on the CIRM Bridges programs. The Shared Research Laboratory also continues to host many outreach activities for members of the public. At these events, stem cell research is explained to a lay audience and members of the public are given an opportunity to see stem cells and learn about the incredible opportunities they provide for developing new treatments for human diseases and injuries. Many groups have visited the facility including high school kids, teachers, patients, patient advocates and caregivers as well as donors and potential donors. Each of these groups is inspirational in their own way and they are also inspired by the science and the potential of stem cell research. On International Stem Cell Awareness Day alone over 200 schoolchildren from local high schools visited the center and learnt about stem cell research. In short, the UC Irvine Regional Human Embryonic Stem Cell Shared Research Laboratory and Stem Cell Techniques Course continues to be an essential element in the success of the Sue and Bill Gross Stem Cell Research Center.

Year 4

During the reporting period the Shared Research Laboratory and Stem cell Technqiues Course continued to be an important and integral component of human embryonic stem cell training on the campus as well as providing important resources for the entire university and local regenerative medicine community. Over 50 students, postdocs and faculty took training courses or other personalized training provided by the facility, greatly enhancing stem cell research on the campus. In addition the provision and maintenance of state of the art equipemnt to the community increased in usage in large part because of the availablity of trained staff who provide expert guidance in using such equipment. The extension of our teaching material to include training in transplantation and imaging technologies provided new opportunities for researchers to learn methodology that will be important in translating basic research discoveries into clinical use. In addition the Shared Research Lab staff continue to provide assistance on an ad hoc basis to researchers from around the campus and in the local community who are either entering the stem cell field or who are experiencing difficulty with their work. For example, one exciting ongoing project represents a collaboration with researchers in the School of Engineering who are developing new methods for detecting, analyzing and treating human heart disease. A second project that reached completion was the development of new materials on which to grow human embryonic stem cells and help maintain them in a pluripotent state. In addition a member of the shared research laboratory staff was part of the organizing committee of the SoCal Flow Summit. Many flow cytometry users from Southern California’s regional research centers, clinical laboratories and leading biomedical industries attended this exciting opportunity for networking, sharing and education with Flow Cytometry colleagues from across the Southern California counties. This two-day meeting included scientific presentations from invited faculty, vendor presentations, poster session and core manager dinner (open to all attendees). The scientific program included six (6) hours of CEUs, available from FloCyte Associates. All meeting attendees registered for this event became members of SoCal Flow Cytometry Association. The meeting was held at the Beckman Center of the National Academies of Science and Engineering and the pratcial sessions were held at Sue and Bill Gross Hall: A CIRM Institute. The meeting brought much attention to the center and to the CIRM supported facilities and equipment. Finally the Shared Research Laboratory continues to be a showcase of the stem cell work ongoing on the campus and highlights the support of the California Institute for Regenerative Medicine. Tours of the laboratory for members of the public, donors and potential donors, highschool teachers and high school students have provided key outreach for the center within the local community and beyond, helped inform the debate about stem cell research and demonstrated the importance of CIRM support for regenerative medicine research.

Year 5

During the last year the Shared Research Lab and Stem Cell Techniques Course has continued to be central to all of the activities of the UC Irvine Sue and Bill Gross Stem Cell Research Center. The Shared Research Lab continues to provide state of the art equipment for researchers across the UC Irvine campus and beyond. The staff supported by the Shared Research Lab grant also continue to provide invaluable technical expertise for many colleagues with their outstanding practical knowledge of the stem cell field. Activities supported by the Shared Research Lab include tours for the public including high school students, training in use of equipment, maintenance of large pieces of equipment, dissemination of new methods and techniques as well as supporting the research of many laboratories whose work is central to the CIRM mission. The Stem Cell Techniques course also continues to be central to the mission of the center and remains in very high demand. Courses are usually completely booked well ahead of time. This year the course has trained another 40 scientists from three different schools across the UC Irvine campus including the Ayala School of Biological Sciences, the Samueli School of Engineering and the School of Medicine. In addition the course was offered to trainees in the Stem Cell Emphasis Track of the Master's Program in Biotechnology that trains students specifically to enter the biotechnology industry. The course is also now being taken by scientists interested in developing induced pluripotent stem cells from patient fibroblasts. The grant supported labs working on induced pluripotent stem cells from patients with Alzheimer's Disease, Huntington's Disease and Paget's Disease. These new areas of investigation indicate the continuing and growing need for support of such resources.

© 2013 California Institute for Regenerative Medicine