Translational Human Embryonic Stem Cell Shared Research Facility

Translational Human Embryonic Stem Cell Shared Research Facility

Funding Type: 
Shared Labs
Grant Number: 
CL1-00504-1.2
Award Value: 
$1,728,111
Stem Cell Use: 
Embryonic Stem Cell
iPS Cell
Status: 
Active
Public Abstract: 
The intent of the proposed shared research facility is to provide a state-wide resource for qualified scientists in California to study human embryonic stem cells (hESC) without federal restrictions. The shared facility will encourage a spirit of collaboration and include laboratories for investigators to culture, collect, store, and analyze hESC, provide necessary services that will be cost-effective and assist with research productivity, and ensure an environment that will facilitate the essential interactions among scientists. This approach will advance the use of hESC for regenerative medicine purposes and aid in developing new technologies and therapies for the treatment of human disease. Using established methods that have proven successful for other collaborative and service-based structures, this facility will encourage scientists to work together and provide the necessary resources to ensure their success. Investigators new to hESC research will benefit greatly by having this facility available because it will have a centralized supportive structure where experienced personnel will provide the necessary assistance and guidance. For those investigators with hESC research experience, new opportunities will be available to work with cell lines that can be obtained but not be used in laboratories that are supported by federal funding. This will greatly expand research programs that are focused, for example, on studying ways to differentiate hESC towards blood cells and vessels for the treatment of disorders such as sickle cell disease and vascular abnormalities associated with heart disease. In addition, regeneration of damaged organs such as the heart, lung, liver, or kidney may require methods to reconstruct these tissues using scaffolds on which to grow the cells. These approaches require the ideas of cell biologists, engineers, biomedical researchers, and clinicians working together, and testing these ideas to ensure the procedures are safe before considering treatments of human patients. Techniques such as those that focus on ways to monitor cells once they are injected into the body will provide a powerful tool to study the outcome of these therapies. A techniques course will be offered to scientists, students, and staff which will result in more laboratories in California working with hESC. The cells are difficult to grow and specialized training is required by personnel that are highly skilled and can provide the necessary information and direction to ensure success. The course is designed to provide qualified applicants the training experiences that will reinforce the basics, and ensure they are able to establish these techniques in their laboratories. The training course will be offered 4 times during the calendar year and include presentations and ‘hands-on’ experiences. Continuing education through the facility will ensure trainees have the necessary support when they return to their respective laboratories.
Statement of Benefit to California: 
The Translational Human Embryonic Stem Cell Shared Research Facility will serve the state and its citizens by providing unparalleled opportunities to investigators, and establish a model for the manner in which researchers throughout California can work together to advance the use of cellular therapies for the treatment of human disease. This facility will remove barriers preventing the transfer of promising stem cell therapies to human patients by connecting people with expertise and new ideas with the resources necessary to develop and to evaluate new technologies and therapies under the necessary conditions before they are assessed in humans. The California community will benefit from the results of this collaborative environment because it will facilitate and advance research findings, promote a culture of sharing, and educate and train a new generation of scientists in human embryonic stem cell research. This approach, the infrastructure and goals of the proposed facility, and the plans for the techniques course will all provide opportunities for scientists, students, fellows, and staff, and increase the number of qualified scientific and medical personnel that will be able to make new discoveries and ultimately improve health care for patients.
Progress Report: 

Year 1

The Translational Shared Research Facility provides a resource for California investigators and trainees to study human embryonic stem cells in an environment of collaboration and to facilitate research needs. The facility includes laboratories for cell culture and analysis, flow cytometry, cell morphology, molecular biology, and provides essential services and training. The facility has a functional website that provides calendar sign-up for facility use.

Year 2

The UC Davis Translational Human Embryonic Stem Cell Shared Research Facility (TSRF) provides a resource for California investigators and trainees to study human embryonic stem cells in a collaborative environment. The TSRF supports research for investigators and provides training to those new to human embryonic stem cell research or those in need of additional guidance. The TSRF includes laboratories for cell culture, flow cytometry and cell sorting, techniques for cellular and molecular analysis of the cells, and essential services and training. Calendar sign-up using a web-based process is available for use of the laboratories and equipment and overall use of the facility.

Year 3

The UC Davis Translational Human Embryonic Stem Cell Shared Research Facility (TSRF) provides a resource for California investigators and trainees to study human embryonic stem cells in a collaborative environment. The TSRF supports research for investigators and trainees at multiple institutions and provides training to those new to human embryonic stem cell research and those in need of additional guidance. The TSRF includes laboratories for cell culture, flow cytometry and cell sorting, techniques for cellular and molecular analysis of the cells, and unique services and training. Calendar sign-up using a web-based process is available for use of the laboratories and equipment in the facility, and a program administrator and scientific staff ensure efficient operation and ease of access, quality training opportunities, and unique techniques to advance human embryonic stem cell research. Established university-approved recharge rates are established for a variety of high quality services in the TSRF.

Year 4

The UC Davis Translational Human Embryonic Stem Cell Shared Research Facility (TSRF) provides a resource for California investigators and trainees to study human embryonic stem cells and related stem and progenitor cell populations in a highly collaborative environment. The TSRF supports research for investigators and provides training and guidance to those new to human embryonic stem cell research or those in need of additional guidance with the culture and study of pluripotent stem cells. The TSRF includes laboratories for cell culture, flow cytometry and cell sorting, techniques for cellular and molecular analysis of the cells, and essential services including assistance with cryopreservation and storage of cell banks for experiments. Calendar sign-up is available for use of the laboratories and equipment within the facility. Examples of services include high quality mouse embryonic feeders (MEFs) on a recharge basis and screened reagents for investigator use. Trainees from CIRM training programs receive training in cell culture and related techniques, and are able to use the facility to pursue their research projects under the guidance of experienced faculty and staff.

Year 5

The Translational Human Embryonic Stem Cell Shared Research Facility is a UC Davis campus-wide service facility dedicated to the study of human stem and progenitor cells. The facility includes three cell culture laboratories, cell sorting, a molecular and histology core, controlled-rate cryopreservation and cell storage, and an infrastructure of experienced personnel that provide services and training in a spectrum of stem cell techniques. This centralized facility provides training opportunities to students at UC Davis, UC Merced, and the California State Universities (e.g., Sacramento, Humboldt) and trainees and investigators at any institution across the State of California. In addition to cell culture, differentiation, and related techniques, the facility has specialized expertise in the labeling of stem and progenitor cells for transplantation and novel tissue engineering approaching using novel scaffolds for repair.

© 2013 California Institute for Regenerative Medicine