Defining Heterogeneity of Human Embryonic Stem Cells

Funding Type: 
SEED Grant
Grant Number: 
RS1-00336
Investigator: 
ICOC Funds Committed: 
$0
oldStatus: 
Closed
Public Abstract: 
Being pluripotent, human embryonic stem cells (hESCs) have the potential to act as a source of cells for regenerative therapies. But before these potential applications of hESCs can be effectively pursued, an understanding of the complex cellular relationships existing within hESC cultures and factors involved in the maintenance of hESC properties is required. hESC lines are known to be morphologically and phenotypically heterogeneous. We propose to select an antibody diversity library directly on live hESCs to identify a panel of monoclonal antibodies that recognize distinct hESC subpopulations, and to further characterize these subpopulations with regards to pluripotency and capacity for self-renewal. These antibodies will be of broad interest to laboratories that are either utilizing and optimizing the existing hESC lines or developing new hECS lines. These antibodies can be used in the future to ensure quality of hECS cultures, develop sublines that are more suitable for regenerative therapy, and identify corresponding antigens for functional studies of hESCs.
Statement of Benefit to California: 
This study aims to develop antibodies that recognize distinct subpopulations of human embryonic stem cells (hESCs), and to further characterize these subpopulations with regards to pluripotency and capacity for self-renewal. These antibodies will be of broad interest to laboratories that are utilizing or optimizing existing hESC lines, or developing new hESC lines. This study benefits California and its citizens because the knowledge and reagents generated can be used in the future to ensure quality of hESC cultures, and develop sublines that are more suitable for regenerative therapy.

© 2013 California Institute for Regenerative Medicine