Identification of a novel common proviral integration site, flit-1, in feline leukemia virus induced thymic lymphoma.

Journal: 
Virology
Publication Year: 
2009
Authors: 
Yasuhito Fujino , Chun-Peng Liao , Yan Shi Zhao , Judong Pan , Lawrence E Mathes , Kathleen A Hayes , Koichi Ohno , Hajime Tsujimoto , Pradip Roy-Burman
Public Summary: 
Scientific Abstract: 
A new proviral integration site for feline leukemia virus (FeLV), termed flit-1, was identified from feline thymic lymphoma. Among 35 FeLV-related tumors examined, 5 of 25 thymic lymphomas demonstrated proviral insertion within flit-1 locus whereas none of four alimentary and five multicentric lymphomas and one T-lymphoid leukemia examined had rearrangement in this region. Extensive sequence analysis has shown that flit-1, which is noncoding, is conserved on human chromosome 12 and mouse chromosome 15. The human and murine homologs of flit-1 are positioned approximately 30-kb upstream to activin-A receptor type II-like 1 (ACVRL1/ALK1) gene. Expression of ACVRL1 mRNA was examined in two of five lymphomas with flit-1 rearrangement and detected in both of the two whereas normal thymuses and seven lymphoid tumors without flit-1 rearrangement had no detectable expression. Therefore, flit-1 appears to represent a novel FeLV proviral common integration domain that may influence lymphomagenesis as insertional mutagenesis.

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