Engineering cell-material interfaces for long-term expansion of human pluripotent stem cells.

Journal: 
Biomaterials
Publication Year: 
2012
Authors: 
Chien-Wen Chang , Yongsung Hwang , Dave Brafman , Thomas Hagan , Catherine Phung , Shyni Varghese
Public Summary: 
This study described development of a synthetic matrix to support self-renewal of human pluripotent stem cells
Scientific Abstract: 
Cost-effective and scalable synthetic matrices that support long-term expansion of human pluripotent stem cells (hPSCs) have many applications, ranging from drug screening platforms to regenerative medicine. Here, we report the development of a hydrogel-based matrix containing synthetic heparin-mimicking moieties that supports the long-term expansion of hPSCs (>/=20 passages) in a chemically defined medium. HPSCs expanded on this synthetic matrix maintained their characteristic morphology, colony forming ability, karyotypic stability, and differentiation potential. We also used the synthetic matrix as a platform to investigate the effects of various physicochemical properties of the extracellular environment on the adhesion, growth, and self-renewal of hPSCs. The observed cellular responses can be explained in terms of matrix interface-mediated binding of extracellular matrix proteins, growth factors, and other cell-secreted factors, which create an instructive microenvironment to support self-renewal of hPSCs. These synthetic matrices, which comprise of "off-the-shelf" components and are easy to synthesize, provide an ideal tool to elucidate the molecular mechanisms that control stem cell fate.

© 2013 California Institute for Regenerative Medicine